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Studies on Serum Fractions

II. Differences in the Antigenic Structure of Different Horse Sera

Hygienic Institute (Eisei-kenkyusho) of the S. M. R. Co., Dairen, Manchuria

Abstract

In the previous report we have shown that by immunizing rabbits with injections of the washed flocculi of diphtheriatoxoid-antitoxin or the specific precipitate of pneumococcic S.S.S. an antiserum reacting with horse serum can be obtained. Using these antisera, great differences in the antigenic structure were found among antitoxic, antibacterial and normal horse-sera. In this report we have added further results of the test with many samples of different normal as well as immune horse-sera and also with sera taken from horses during the course of immunization.

The designation of "precipitin-versus-antitoxin" (abbreviation: P.V.At.) for the antiserum prepared with flocculi of diphtheriatoxoid-antitoxin and of "precipitin-versus-antibacterial antibody" (abbreviation: P.V.Ab.) for that prepared with a specific precipitate of pneumococcic S.S.S. was used throughout the text and in the tables. For convenience and also for some other reasons, we designated the fraction giving rise to the precipitation against "precipitin-versus-antitoxin" as "A-fraction" and that causing the primary zone against "precipitin-versus-antibacterial antibody" as "B-fraction" or "Felton-fraction."