The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chen, J.
Right arrow Articles by Fruman, D. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chen, J.
Right arrow Articles by Fruman, D. A.
Right arrowPubmed/NCBI databases
*Gene*GEO Profiles
*HomoloGene*UniGene
*Substance via MeSH
The Journal of Immunology, 2006, 176: 2711-2721.
Copyright © 2006 by The American Association of Immunologists

FOXO Transcription Factors Cooperate with {delta}EF1 to Activate Growth Suppressive Genes in B Lymphocytes1

Jing Chen*, Isharat Yusuf*, Hilde-Marie Andersen* and David A. Fruman2,{dagger}

* Department of Molecular Biology and Biochemistry, and {dagger} Center for Immunology, University of California, Irvine, CA 92697

Forkhead transcription factors regulate many aspects of lymphocyte development and function. The FOXO subgroup of Forkhead factors opposes proliferation and survival, and FOXO inactivation is an important outcome of Ag receptor signaling. FOXO activity at target promoters is modulated by other transcription factors in a manner dependent on cell type and external stimulus. We have investigated the mechanisms by which FOXO proteins activate the promoters of two target genes in murine B lymphocytes, Ccng2 (encoding cyclin G2) and Rbl2 (p130), each of which has been implicated in cell cycle arrest. FOXO proteins bound directly to both promoters in vitro and in vivo, augmented transcriptional activity in reporter assays, and increased expression of the endogenous genes. Each of the promoter sequences has consensus binding sites for the {delta}EF1 transcription factor, previously shown to either repress or activate different promoters. {delta}EF1 bound to the Ccng2 and Rbl2 promoters in vitro and in vivo and increased reporter activity as well as endogenous mRNA levels for these genes. Strikingly, {delta}EF1 synergized with FOXO proteins to strongly activate transcription from both promoters. Coexpression of {delta}EF1 enhanced FOXO-induced cell cycle arrest in B lymphoma cells. These findings establish a novel mechanism of FOXO function at target promoters: cooperation with {delta}EF1.




This article has been cited by other articles:


Home page
 Int ImmunolHome page
R. M. Hinman, W. A. Nichols, T. M. Diaz, T. D. Gallardo, D. H. Castrillon, and A. B. Satterthwaite
Foxo3-/- mice demonstrate reduced numbers of pre-B and recirculating B cells but normal splenic B cell sub-population distribution
Int. Immunol., July 1, 2009; 21(7): 831 - 842.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. Fabre, F. Carrette, J. Chen, V. Lang, M. Semichon, C. Denoyelle, V. Lazar, N. Cagnard, A. Dubart-Kupperschmitt, M. Mangeney, et al.
FOXO1 Regulates L-Selectin and a Network of Human T Cell Homing Molecules Downstream of Phosphatidylinositol 3-Kinase
J. Immunol., September 1, 2008; 181(5): 2980 - 2989.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
T. Hidaka, S. Nakahata, K. Hatakeyama, M. Hamasaki, K. Yamashita, T. Kohno, Y. Arai, T. Taki, K. Nishida, A. Okayama, et al.
Down-regulation of TCF8 is involved in the leukemogenesis of adult T-cell leukemia/lymphoma
Blood, July 15, 2008; 112(2): 383 - 393.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
T. Pew, M. Zou, D. R. Brickley, and S. D. Conzen
Glucocorticoid (GC)-Mediated Down-Regulation of Urokinase Plasminogen Activator Expression via the Serum and GC Regulated Kinase-1/Forkhead Box O3a Pathway
Endocrinology, May 1, 2008; 149(5): 2637 - 2645.
[Abstract] [Full Text] [PDF]

Home page
 Int ImmunolHome page
I. Yusuf, M. G. Kharas, J. Chen, R. Q. Peralta, A. Maruniak, P. Sareen, V. W. Yang, K. H. Kaestner, and D. A. Fruman
KLF4 is a FOXO target gene that suppresses B cell proliferation
Int. Immunol., May 1, 2008; 20(5): 671 - 681.
[Abstract] [Full Text] [PDF]

Home page
 Molecular Cancer TherapeuticsHome page
X.-F. Le, A. S. Arachchige-Don, W. Mao, M. C. Horne, and R. C. Bast Jr.
Roles of human epidermal growth factor receptor 2, c-jun NH2-terminal kinase, phosphoinositide 3-kinase, and p70 S6 kinase pathways in regulation of cyclin G2 expression in human breast cancer cells
Mol. Cancer Ther., November 1, 2007; 6(11): 2843 - 2857.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
K. L. Good and S. G. Tangye
Decreased expression of Kruppel-like factors in memory B cells induces the rapid response typical of secondary antibody responses
PNAS, August 14, 2007; 104(33): 13420 - 13425.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
R. M. Hinman, J. N. Bushanam, W. A. Nichols, and A. B. Satterthwaite
B Cell Receptor Signaling Down-Regulates Forkhead Box Transcription Factor Class O 1 mRNA Expression via Phosphatidylinositol 3-Kinase and Bruton's Tyrosine Kinase
J. Immunol., January 15, 2007; 178(2): 740 - 747.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
M. G. Kharas, I. Yusuf, V. M. Scarfone, V. W. Yang, J. A. Segre, C. S. Huettner, and D. A. Fruman
KLF4 suppresses transformation of pre-B cells by ABL oncogenes
Blood, January 15, 2007; 109(2): 747 - 755.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 2006 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 2006 by The American Association of Immunologists, Inc. All rights reserved.